Yeast permeabilization as a tool for measurement of in situ enzyme activity: localization of alkaline phosphatase.
نویسندگان
چکیده
The biochemical and ultracytochemical localization of alkaline phosphatases in permeabilized cells of Saccharomyces cerevisiae 257 has been studied. The treatment with non-ionic surfactant Triton X-100 allows the penetration of the substrate into intact yeast cells and thus provides detailed detection of the enzyme activity in ultracytochemical studies.
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عنوان ژورنال:
- Zeitschrift fur Naturforschung. C, Journal of biosciences
دوره 53 5-6 شماره
صفحات -
تاریخ انتشار 1998